AAV Neutralizing Antibodies

Find more information on the neutralizing activity of our antibodies for the corresponding AAV serotypes below.

A20 (AAV2 intact particles):

The A20 antibody (Cat. No. 61055) was tested for neutralization using different antibody concentrations. After the HeLa cells were seeded in a 96-well plate they were incubated for 48h with theAAV2 particles containing a GFP reporter and the indicated concentration of the A20 antibody.

The microscopic analysis of the cells clearly demonstrates the neutralizing activity of the A20 antibody by decreasing GFP expression with increasing antibody concentration.   

Find more information on the neutralizing activity of the A20 antibody in the following publications:

Moskalenko, M. et al. Epitope Mapping of Human Anti-Adeno-Associated Virus Type 2 Neutralizing Antibodies: Implications for Gene Therapy and Virus Structure. Jounal Virol.74, 1761–1766 (2000).

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol.74, 9281–93 (2000).

 

A20R (AAV2 intact particles):

The A20R antibody (Cat. No. 610298S) was tested for neutralization using different antibody concentrations. After the HeLa cells were seeded in a 96-well plate they were incubated for 48h with theAAV2 particles containing a GFP reporter and the indicated concentration of the A20R antibody.

The microscopic analysis of the cells clearly demonstrates the neutralizing activity of the A20R antibody by decreasing GFP expression with increasing antibody concentration.   

ADK5a (AAV5 intact particles):

The ADK5a antibody (Cat. No. 610148) was tested for neutralization using different antibody concentrations. After the HeLa cells were seeded in a 96-well plate they were incubated for 48h with theAAV5 particles containing a GFP reporter and the indicated concentration of the ADK5a antibody.

The microscopic analysis of the cells clearly demonstrates the neutralizing activity of the ADK5a antibody by decreasing GFP expression with increasing antibody concentration.   

 

 

 

ADK5b (AAV5 intact particles):

The ADK5b antibody (Cat. No. 610149) was tested for neutralization using different antibody concentrations. After the HeLa cells were seeded in a 96-well plate they were incubated for 48h with theAAV5 particles containing a GFP reporter and the indicated concentration of the ADK5b antibody.

The microscopic analysis of the cells clearly demonstrates the neutralizing activity of the ADK5b antibody by decreasing GFP expression with increasing antibody concentration.   

Find more information on the neutralizing activity of the ADK5b antibody in the following publication:

Tseng, Y.-S. et al. Adeno-Associated Virus Serotype 1 (AAV1)-and AAV5-Antibody Complex Structures Reveal Evolutionary Commonalities in Parvovirus Antigenic Reactivity. J. Virol. 89, 1794–1808 (2015).

ADK6 (AAV6 intact particles):

The ADK6 antibody (Cat. No. 610159) was tested for neutralization using different antibody concentrations. After the HeLa cells were seeded in a 96-well plate they were incubated for 48h with theAAV6 particles containing a GFP reporter and the indicated concentration of the ADK6 antibody.

The microscopic analysis of the cells clearly demonstrates the neutralizing activity of the ADK6 antibody by decreasing GFP expression with increasing antibody concentration.   

Find more information on the neutralizing activity of the ADK6 antibody in the following publication:

Bennett, A. D. et al. AAV6 K531 serves a dual function in selective receptor and antibody ADK6 recognition. Virology518, 369-376 (2018)

ADK8 (AAV8 intact particles):

The ADK8 antibody (Cat. No. 610160) was tested for neutralization using different antibody concentrations. After the HeLa cells were seeded in a 96-well plate they were incubated for 48h with theAAV8 particles containing a GFP reporter and the indicated concentration of the ADK8 antibody.

The microscopic analysis of the cells clearly demonstrates the neutralizing activity of the ADK8 antibody by decreasing GFP expression with increasing antibody concentration.   

Find more information on the neutralizing activity of the ADK8 antibody in the following publications:

Gurda, B. L. et al. Mapping a Neutralizing Epitope onto the Capsid of Adeno-Associated Virus Serotype 8. J. Virol.86, 7739–7751 (2012).

Raupp, C. et al. The Threefold Protrusions of Adeno-Associated Virus Type 8 Are Involved in Cell Surface Targeting as Well as Postattachment Processing. J. Virol.86, 9396–9408 (2012).

Sonntag, F. et al. The assembly activating protein (AAP) promotes capsid assembly of different AAV serotypes. J. Virol. 85, 12686-97 (2011)

ADK9 (AAV9 intact particles):

The ADK9 antibody (Cat. No. 690162) was tested for neutralization using different antibody concentrations. After the HeLa cells were seeded in a 96-well plate they were incubated for 48h with theAAV9 particles containing a GFP reporter and the indicated concentration of the ADK9 antibody.

The microscopic analysis of the cells clearly demonstrates the neutralizing activity of the ADK9 antibody by decreasing GFP expression with increasing antibody concentration.   

Find more information on the neutralizing activity of the ADK9 antibody in the following publications:

Sonntag, F. et al. The assembly activating protein (AAP) promotes capsid assembly of different AAV serotypes. J. Virol. 85, 12686-97 (2011)

Tseng, Y.S. et al. Generation and characterization of anti-Adeno-associated virus serotype 8 (AAV8) and anti-AAV9 monoclonal antibodies. J. Virol. Meth. 236, 105-110 (2016)

Varadi, K. et al. Novel random peptide libraries displayed on AAV serotype 9 for selection of endothelial cell-directed gene transfer vectors. Gene Ther. 19, 800-9 (2012).

 

ADK9-1R (AAV9 intact particles):

The ADK9-1R antibody (Cat. No. 610178) was tested for neutralization using different antibody concentrations. After the HeLa cells were seeded in a 96-well plate they were incubated for 48h with theAAV9 particles containing a GFP reporter and the indicated concentration of the ADK9-1R antibody.

The microscopic analysis of the cells clearly demonstrates the neutralizing activity of the ADK9-1R antibody by decreasing GFP expression with increasing antibody concentration.