Antibodies for the Detection of Adeno-Associated Viruses (AAV)

AAV antibodies are suitable for the characterization of different stages of an AAV infection and are effective in the analysis of the AAV assembly process. There are two categories of AAV antibodies: 

(i)    AAV capsid protein antibodies that specifically react with denatured AAV capsid proteins and thereby recognize the linear epitopes of the viral capsids.

(ii)    AAV particle antibodies that specifically react with intact AAV particles, i.e. empty and full capsids and thereby recognize a conformational epitope of the assembled capsids of certain serotypes. 

AAV Capsid Protein Antibodies

The AAV capsid protein antibodies recognize linear epitopes of the viral capsid proteins that consist of neighboring amino acids. These epitopes have high sequence similarity and therefore the antibodies detect the viral capsid proteins VP1, VP2 and VP3 across the serotypes. The antibodies are useful to study the expression levels of VP1, VP2 and VP3 in western blot analyses, in order to determine the protein localizations or protein-protein interactions by affinity chromatography, immunoprecipitation or immunofluorescence.

For more information on our specific AAV capsid protein antibodies, please see our VP1, VP1/VP2 and VP1/VP2/VP3 antibodies below.

Some of the following antibodies are also available in sample sizes of 10µg (lyophilized). 

AAV Particle Antibodies

The AAV particle antibodies recognize conformational epitopes of the viral capsid proteins that are only present on fully assembled capsids. These epitopes consist of distant amino acids either of the same protein or of different proteins that are in close proximity, and they might vary across the different serotypes. However, for some of the antibodies some cross-reactivity is known (for more information, please see the specific AAV particle antibodies by AAV serotype below). The AAV particle antibodies are useful for the following processes: 

  • to study fully assembled and intact capsids by dot blot analyses
  • to quantify the intact viral capsids (empty and full) by ELISA
  • to detect neutralizing antibodies in patients serum
  • to determine protein-protein interaction or to purify virus preparations by affinity chromatography or immunoprecipitations
  • to analyze virus particle localizations by immunofluorescence.

Some of the following antibodies are also available in sample sizes of 10µg (lyophilized). 

Potential Binding Sites of the ADK1a/1b Antibody

The ADK1a antibody used for the AAV1 Titration ELISA as well as the ADK1b specifically recognize assembled AAV1 capsids, hence epitopes that result from a specific conformational assembly of the capsid proteins VP1, VP2 and VP3. In the publication cited below the contact sites and footprint residues identified for ADK1a and ADK1b are described and the results are summarized in table 1. Multiple contact sites and footprint residues have been identified, that are very likely to be part of the binding site. The amino acids of each binding site are located in different parts of the protein chains and are recognized as the epitope of the antibody only in the assembled capsid where they are in close proximity to each other and in the correct conformation.

Neutralizing activity of the ADK1 Antibody

For AAV1 the neutralization epitope has been identified and might give an additional indication about the binding residues of the neutralizing antibody ADK1 used for the corresponding AAV1 Titration ELISAs.

anti-AAV1 (intact particle) mouse monoclonal, ADK1a, lyophilized, purifiedCat. No. 610150
anti-AAV1 (intact particle) mouse monoclonal, ADK1a, lyophilized, purified, sampleCat. No. 610150S
anti-AAV1 (intact particle) mouse monoclonal, ADK1a, Biotin ConjugateCat. No. 615150
AAV1 is also recognized by ADK8 (see AAV8). 

 

 

Potential Binding Sites of the A20 Antibody

The A20 and A20R antibodies used for the AAV2 Titration ELISAs (PRATV & PRAAV2R) specifically recognize assembled AAV2 capsid, hence epitopes that result from a specific conformational assembly of the capsid proteins VP1, VP2 and VP3. Several publications cited below describe the analysis of binding sites for the A20 antibody using different techniques. Multiple amino acids have been identified, that are very likely to be part of the binding site, especially those that were identified with more than one method (green boxes). The amino acids of each binding site are located in different parts of the protein chains and are recognized as the epitope of the antibody only in the assembled capsid where they are in close proximity to each other and in the correct conformation.

Neutralizing Activity of the A20 & A20R Antibody

The A20 (Cat. No.61055) and the A20R antibody (Cat. No.610298S) were tested for neutralization using different antibody concentrations. After the HeLa cells were seeded in a 96-well plate they were incubated for 48h with the AAV2 particles containing a GFP reporter and the indicated concentration of the A20 or A20R antibody, respectively.

The microscopic analysis of the cells clearly demonstrates the neutralizing activity of the A20 and A20R antibody by decreasing GFP expression with increasing antibody concentration.   

 
anti-AAV2 (intact particle) mouse monoclonal, A20, lyophilized, purifiedCat. No. 61055
anti-AAV2 (intact particle) mouse monoclonal, A20, lyophilized, purified, sampleCat. No. 61055S

anti-AAV2 (intact particle) mouse monoclonal, A20, Biotin Conjugate

Cat. No. 61555
anti-AAV2 (intact particle) mouse monoclonal, A20, supernatantCat. No. 65155
anti-AAV2 (intact particle) mouse recombinant, A20R, lyophilized, purifiedCat. No. 610298
anti-AAV2 (intact particle) mouse recombinant, A20R, lyophilized, purified, sampleCat. No. 610298S
anti-AAV2 (intact particle) mouse recombinant, A20R, Biotin ConjugateCat. No. 615298
AAV3 is recognized by A20 (see AAV2) and ADK8 (see AAV8). 
anti-AAV4 (intact particle) mouse monoclonal, ADK4, lyophilized, purifiedCat. No. 610147
anti-AAV4 (intact particle) mouse monoclonal, ADK4, lyophilized, purified, sampleCat. No. 610147S
anti-AAV4 (intact particle) mouse monoclonal, ADK4, Biotin ConjugateCat. No. 615147  

Potential Binding Sites of the ADK5a/5b Antibody

The ADK5a  antibody used for the AAV5 Titration ELISA as well as the ADK5b specifically recognize assembled AAV5 capsids, hence epitopes that result from a specific conformational assembly of the capsid proteins VP1, VP2 and VP3. In the publication cited below the contact sites and footprint residues identified for ADK5a and ADK5b are described and the results are summarized in table 1. Multiple contact sites and footprint residues have been identified, that are very likely to be part of the binding site. The amino acids of each binding site are located in different parts of the protein chains and are recognized as the epitope of the antibody only in the assembled capsid where they are in close proximity to each other and in the correct conformation.

Neutralizing Activity of the ADK5a & ADK5b Antibody

The ADK5a (Cat. No. 610148) and the ADK5b antibody (Cat. No. 610149) were tested for neutralization using different antibody concentrations. After the HeLa cells were seeded in a 96-well plate they were incubated for 48h with the AAV5 particles containing a GFP reporter and the indicated concentration of the ADK5a or ADK5b antibody, respectively.

The microscopic analysis of the cells clearly demonstrates the neutralizing activity of the ADK5a or ADK5b antibody by decreasing GFP expression with increasing antibody concentration.  

 

 

anti-AAV5 rabbit polyclonal, serumCat. No. 610137
anti-AAV5 (intact particle) mouse monoclonal, ADK5a, lyophilized, purifiedCat. No. 610148
anti-AAV5 (intact particle) mouse monoclonal, ADK5a, lyophilized, purified, sampleCat. No. 610148S
anti-AAV5 (intact particle) mouse monoclonal, ADK5a, Biotin ConjugateCat. No. 615148
anti-AAV5 (intact particle) mouse monoclonal, ADK5b, lyophilized, purifiedCat. No. 610149
anti-AAV5 (intact particle) mouse monoclonal, ADK5b, lyophilized, purified, sampleCat. No. 610149S

 

 

Neutralizing Activity of the ADK6 Antibody

The ADK6 antibody (Cat. No. 610159) was tested for neutralization using different antibody concentrations. After the HeLa cells were seeded in a 96-well plate they were incubated for 48h with theAAV6 particles containing a GFP reporter and the indicated concentration of the ADK6 antibody.

The microscopic analysis of the cells clearly demonstrates the neutralizing activity of the ADK6 antibody by decreasing GFP expression with increasing antibody concentration.   

Find more information on the neutralizing activity of the ADK6 antibody in the following publication:

Bennett, A. D. et al. AAV6 K531 serves a dual function in selective receptor and antibody ADK6 recognition. Virology518, 369-376 (2018)

 

anti-AAV6 (intact particle) mouse monoclonal, ADK6, lyophilized, purified Cat. No. 610159
anti-AAV6 (intact particle) mouse monoclonal, ADK6, lyophilized, purified, sample Cat. No. 610159S
anti-AAV6 (intact particle) mouse monoclonal, ADK6, biotin conjugate Cat. No. 615159
AAV6 is also recognized by ADK1 (see AAV1).
AAV7 is recognized by ADK8 (see AAV8).  

 

 

Potential Binding Sites of the ADK8 Antibody

The ADK8 antibody used for the AAV8 Titration ELISA (PRAAV8) specifically recognizes assembled AAV8 capsid, hence epitopes that result from a specific conformational assembly of the capsid proteins VP1, VP2 and VP3. The publication cited below shows the result of the Cryo-EM analysis of binding sites for the ADK8 antibody. In this publication one potential epitope has been identified which is likely to be part of the ADK8 binding site.

Neutralizing Activity of the ADK8 Antibody

The ADK8 antibody (Cat. No. 610160) was tested for neutralization using different antibody concentrations. After the HeLa cells were seeded in a 96-well plate they were incubated for 48h with theAAV8 particles containing a GFP reporter and the indicated concentration of the ADK8 antibody.

The microscopic analysis of the cells clearly demonstrates the neutralizing activity of the ADK8 antibody by decreasing GFP expression with increasing antibody concentration.   

Find more information on the neutralizing activity of the ADK8 antibody in the following publications:

Gurda, B. L. et al. Mapping a Neutralizing Epitope onto the Capsid of Adeno-Associated Virus Serotype 8. J. Virol.86, 7739–7751 (2012).

Raupp, C. et al. The Threefold Protrusions of Adeno-Associated Virus Type 8 Are Involved in Cell Surface Targeting as Well as Postattachment Processing. J. Virol.86, 9396–9408 (2012).

Sonntag, F. et al. The assembly activating protein (AAP) promotes capsid assembly of different AAV serotypes. J. Virol. 85, 12686-97 (2011)

 

anti-AAV8 (intact particle) mouse monoclonal, ADK8, lyophilized, purifiedCat. No. 610160
anti-AAV8 (intact particle) mouse monoclonal, ADK8, lyophilized, purified, sampleCat. No. 610160S
anti-AAV8 (intact particle) mouse monoclonal, ADK8, biotin conjugateCat. No. 615160
anti-AAV8 (intact particle) mouse monoclonal, ADK8, supernatantCat. No. 651160
anti-AAV8/9 (intact particle) mouse monoclonal, ADK8/9, supernatantCat. No. 651161

 

 

 

 

Neutralizing Activity of the ADK9 & ADK9-1R Antibody

The ADK9 (Cat. No. 690162) and the ADK9-1R antibody (Cat. No. 610178) were tested for neutralization using different antibody concentrations. After the HeLa cells were seeded in a 96-well plate they were incubated for 48h with the AAV9 particles containing a GFP reporter and the indicated concentration of the ADK9 or the ADK9-1R antibody, respectively.

The microscopic analysis of the cells clearly demonstrates the neutralizing activity of the ADK9 and ADK9-1R antibody by decreasing GFP expression with increasing antibody concentration.   

 

Neutralization epitope of the ADK9 Antibody

For AAV9 the neutralization epitope has been identified and might give an additional indication about the binding residues of the neutralizing antibody ADK9 used for the corresponding AAV9 Titration ELISAs.

 

anti-AAV-8/9 (intact particle) mouse monoclonal, ADK8/9 supernatantCat. No. 651161
anti-AAV9 (intact particle) mouse monoclonal, ADK9, supernatantCat. No. 651162
anti-AAV9 (intact particle) mouse monoclonal, ADK9, liquid, purifiedCat. No. 690162
anti-AAV9 (intact particle) mouse monoclonal, ADK9, liquid, purified, sampleCat. No. 690162S

anti-AAV9 (intact particle) mouse recombinant, ADK9-1R, lyophilized, purified

Cat. No. 610178

anti-AAV9 (intact particle) mouse recombinant, ADK9-1R, lyophilized, purified, sample

Cat. No. 610178S
anti-AAV9 (intact particle) mouse monoclonal, ADK9, biotin conjugateCat. No. 615162
AAVrh10 is recognized by ADK8 (see AAV8).  
AAV12 is recognized by ADK1a (see AAV1).  
AAVrh74 is recognized by ADK8 (see AAV8) and ADK8/9 (see AAV8 & AAV9).  
Anc80 is recognized by A20 (see AAV2) and ADK8 (see AAV8).  

Anti-AAV Replicase proteins

The antibodies react with selected Rep (replicase) proteins in human AAV-infected cells.

anti-AAV2 Replicase mouse monoclonal, 76.2, lyophilized, purifiedCat. No. 61073
anti-AAV2 Replicase mouse monoclonal, 226.7, supernatantCat. No. 65172
anti-AAV2 Replicase mouse monoclonal, 259.5, lyophilized, purifiedCat. No. 61071
anti-AAV2 Replicase mouse monoclonal, 303.9, lyophylized, purifiedCat. No. 61069